Anti-GRP78 BiP antibody ab21685 from Abcam

Product Specs
ItemAnti-GRP78 BiP antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab21685
Antibody TypePrimary
ClonalityPolyclonal
IsotypeIgG
HostRabbit
Quantity100 UG
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
Concentration0.2 mg/ml
Gene NameHSPA5
ReactivityChinese hamster, Human, Mouse, Rat, Recombinant fragment
TargetGRP78 BiP

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Treatment with β-sitosterol ameliorates the effects of cerebral ischemia/reperfusion injury by suppressing cholesterol overload, endoplasmic reticulum stress, and apoptosisNeural Regeneration ResearchMarch 1, 2024Xiuling Tang, Tao Yan, Saiying Wang, Qingqing Liu, Qi Yang, Yongqiang Zhang, Yujiao Li, Yumei Wu, Shuibing Liu, Yulong Ma, et al.After blocking, the membranes were incubated with rabbit polyclonal anti-GRP78/Bip (1:1000, Abcam, Cat# ab21685, RRID: AB_2119834), rabbit polyclonal anti-Caspase 12 (1:1000, Abcam, Cat# ab62484, RRID: AB_955729),

(2) Chronic stress-induced anxiety-like behavior, hippocampal oxidative, and endoplasmic reticulum stress are reversed by young plasma transfusion in aged adult ratsIran J Basic Med SciJanuary 1, 2024Arshad Ghaffari-Nasab, Gonja Javani, Fereshteh Farajdokht, Mohammad Reza Alipour, Gisou Mohaddestemperature with primary antibodies against CHOP (1:500, Santa Cruz, sc-7351), GRP78 (1:500, Abcam, ab21685), and β-Actin (1:500, Santa Cruz, sc-47778) in the antibody buffer containing 1% (w/v) nonfat dry milk

(3) Allelic effects on uromodulin aggregates drive autosomal dominant tubulointerstitial kidney diseaseEMBO Molecular MedicineDecember 7, 2023Guglielmo Schiano, Jennifer Lake, Marta Mariniello, Céline Schaeffer, Marianne Harvent, Luca Rampoldi, Eric Olinger, Olivier Devuystat 4°C with 50 μl protein G‐Sepharose beads pre‐conjugated with 3 μl of rabbit anti GRP78 antibody (ab21685, Abcam) or with 6 μl of sheep anti‐uromodulin antibody (K90071C, Meridian Life Science Inc., Cincinnati,

(4) Sustained activation of 12/15 lipoxygenase (12/15 LOX) contributes to impaired renal recovery post ischemic injury in male SHR compared to femalesMol MedDecember 4, 2023Riyaz Mohamed, Jennifer C. SullivanProtein expression was determined with two-color immunoblots using primary antibodies to GPR78 (cat# ab21685; 1:1000 dilution, Abcam, USA), phosphorylated PERK (cat# 3179 S; 1:1000 dilution, Cell Signaling Technology,

(5) Cytotoxic pathways activated by multifunctional thiosemicarbazones targeting sigma-2 receptors in breast and lung carcinoma cellsPharmacological ReportsDecember 1, 2023Joanna Kopecka, Alessandra Barbanente, Daniele Vitone, Fabio Arnesano, Nicola Margiotta, Paola Berchialla, Mauro Niso, Chiara Riganti, Carmen Abate4 °C. 20 μg protein extracts were subjected to SDS–PAGE and probed with the antibodies for: GRP78 (#ab21685), ATF6 (#ab122897), IRE-1 (#ab37073), PERK (#ab65142), actin (#ab1801) (all from Abcam, Cambridge, UK),

(6) Copper drives prion protein phase separation and modulates aggregationScience AdvancesNovember 3, 2023Mariana Juliani do Amaral, Satabdee Mohapatra, Aline Ribeiro Passos, Taiana Sousa Lopes da Silva, Renato Sampaio Carvalho, Marcius da Silva Almeida, Anderson Sá Pinheiro, Susanne Wegmann, Yraima Cordeiroparaformaldehyde) were Triton X-100–permeabilized cells followed by incubation with anti-GRP78/BIP (1:200; Abcam ab21685) in blocking solution [3% normal goat serum in PBS (pH 7.4)]. Subsequently, the secondary antibody (1:1000;

(7) Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryosFrontiers in Cell and Developmental BiologyOctober 30, 2023Wen-Jie Jiang, Song-Hee Lee, Geun Heo, Hak Jae Chung, Eun Seok Cho, Soo Jin Sa, Shinichi Hochi, Xiang-Shun Cui20339-1-AP, Proteintech), rabbit anti-PINK1 (1:100, 23274-1-AP, Proteintech), rabbit anti-GRP78 (1:100, ab21685, Abcam), rabbit anti-calnexin (1:100, ab22595, Abcam), light chain 3 (LC3) (1:100, NB100-2220, Novus biologicals

(8) IGF2BP2‑dependent STIM1 inhibition protects against LPS‑induced pneumonia in vitro by alleviating endoplasmic reticulum stress and the inflammatory responseExperimental and Therapeutic MedicineOctober 26, 2023Wei Zhou, Qigang Dai, Ning Su, Zhihui Liu, Jinxing HuAbcam), Bax (1:1,000; cat. no. ab32503; Abcam), glucose-regulated protein 78 (GRP78; 1:1,000; cat. no. ab21685; Abcam), activating transcription factor 6 (ATF6; 1:1,000; cat. no. ab227830; Abcam), C/EBP homologous

(9) Fine particulate matter promotes airway inflammation and mucin production by activating endoplasmic reticulum stress and the IRE1α/NOD1/NF‑κB pathwayInternational journal of molecular medicineOctober 1, 2023Hu L, Xu C, Tang X, Yu S, Wang L, Li Q, Zhou X.Technology, Ltd. Rabbit anti-human glucose-regulated protein 78 (GRP78) polyclonal antibody (cat. no. ab21685), and rabbit anti-human PERK monoclonal antibody (cat. no. ab229912) were obtained from Abcam. Rabbit

(10) Combination of RUNX1 inhibitor and gemcitabine mitigates chemo‐resistance in pancreatic ductal adenocarcinoma by modulating BiP/PERK/eIF2α-axis-mediated endoplasmic reticulum stressJournal of Experimental & Clinical Cancer ResearchSeptember 11, 2023Chunhua She, Chao Wu, Weihua Guo, Yongjie Xie, Shouyi Li, Weishuai Liu, Chao Xu, Hui Li, Pei Cao, Yanfang Yang, et al.Antibodies used for Western blot were as follow: RUNX1 (ab23980, Abcam), RUNX1 (ab189172, Abcam), BiP (ab21685, Abcam), p-IRE1α (ab48187,S724, Abcam), ATF6 (ab227830, Abcam), XBP-1(sc-8015, Santa cruz) and the antibodies

(11) GRP78 inhibitor HA15 increases the effect of Bortezomib on eradicating multiple myeloma cells through triggering endoplasmic reticulum stressHeliyonSeptember 2, 2023Yirong Chen, Yuchen Tao, Kexin Hu, Jiahui Luantibodies against Bax (1:3500, ab32503, Abcam, CA, USA), Bcl2 (1:1500, ab182858, Abcam), GRP78 (1:1000, ab21685, Abcam), ATF4 (1:1000, ab216839, Abcam), CHOP (1:1000, ab11419, Abcam), XBP1 (1:1500, ab37152, Abcam),

(12) Oxidative and Endoplasmic Reticulum Stress Represent Novel Therapeutic Targets for ChoroideremiaAntioxidants (Basel)August 30, 2023Hajrah Sarkar, Manuela Lahne, Neelima Nair, Mariya MoosajeeWestern Blot Samples were analysed using western blot, as previously described [12], using anti-BIP (1:1000; Abcam ab21685, Cambridge, UK) overnight at 4 °C. The membrane was stripped and re-probed with anti-β-actin for 2 h

(13) SOD1G93A Astrocyte-Derived Extracellular Vesicles Induce Motor Neuron Death by a miRNA-155-5p-Mediated MechanismASN NeuroAugust 30, 2023Soledad Marton, Ernesto Miquel, Joaquín Acosta-Rodríguez, Santiago Fontenla, Gabriela Libisch, Patricia Cassinade Ciencias, UdelaR, 1:500 (Palacios et al., 2010); and rabbit polyclonal anti-BIP, 1:1,000 (Abcam, ab21685, RRID: AB_2119834). Afterward, the membranes were washed in TBS-T and incubated for 60 min at room temperature

(14) 4-PBA inhibits endoplasmic reticulum stress to improve autophagic flux in the treatment of protamine/lipopolysaccharide-induced interstitial cystitis in ratsScientific ReportsAugust 28, 2023Li Jia, Zhu Jingzhen, Yang Xinliang, Sun Bishao, Luo Xin, Zheng Ji, Fang Zhenqiangextracting total protein from bladder tissue. Western blot was used to detect the expression of GRP78(Abcam, ab21685), LC3I/II(Cell Signaling Technology, #12741),Beclin1(Cell Signaling Technology, #3495), P62(Abcam, ab109012),

(15) N-linked glycosylation is essential for anti-tumor activities of KIAA1324 in gastric cancerCell Death & DiseaseAugust 23, 2023Rebecca Yun, Eunji Hong, Junil Kim, Bora Park, Staci Jakyong Kim, Bona Lee, Yong Sang Song, Seong-Jin Kim, Sujin Park, Jin Muk Kang#4668), anti-JNK (Cell Signaling Technology, #9252), anti-GRP78 (Abcam, Cambridge, United Kingdom, ab21685), anti-PDI, anti-β-actin (Abcam, ab8227), anti-KIAA1324 (Sigma, Missouri, USA, SAB2900912) and α-tubulin

(16) SIRT6 deficiency in endothelial cells exacerbates oxidative stress by enhancing HIF1α accumulation and H3K9 acetylation at the Ero1α promoterClinical and Translational MedicineAugust 20, 2023Zhenyang Guo, Xueting Yu, Shuang Zhao, Xin Zhong, Dong Huang, Runyang Feng, Peng Li, Zheyan Fang, Yiqing Hu, Zhentao Zhang, et al.Thermo Fisher, USA), p‐PERK (PA5‐102853, Thermo Fisher, USA), PERK (PA5‐120620, Thermo Fisher, USA), BIP (ab21685, Abcam, USA), p‐eif2α (3398S, CST, USA), eif2α (5324S, CST, USA), CHOP (2895S, CST, USA), CC3 (19677‐1‐AP,

(17) FOXA2 attenuates lipopolysaccharide‑induced pneumonia by inhibiting the inflammatory response, oxidative stress and apoptosis through blocking of p38/STAT3 signalingExperimental and Therapeutic MedicineAugust 17, 2023Zhibin Xue, Yinglin Li, Shiji Xiao, Hanqing Zhang, Jianzhang XuCell Signaling Technology, Inc.), STAT3 (cat. no. ab109085; 1:1,000; Abcam), glucose-regulated protein 78 (GRP78; cat. no. ab21685; 1:1,000; Abcam), CHOP (cat. no. 5554; 1:1,000; Cell Signaling Technology, Inc.), X-box binding protein

(18) Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolismJCI insightAugust 3, 2023Abdon B, Liang Y, da Luz Scheffer D, Torres M, Shrestha N, Reinert RB, Lu Y, Pederson B, Bugarin-Lapuz A, Kersten S, et al.anti-SEL1L (made in house, ref. 20; 1:10,000); anti-HRD1 (Proteintech, 13473-1-AP; 1:5000), anti-BiP (Abcam, ab21685; 1:1000), anti-calnexin (Enzo Life Sciences, ADI-SPA-860; 1:1000), anti-PDI (Enzo Life Sciences, ADI-SPA-890;

(19) Piezo1 suppression reduces demyelination after intracerebral hemorrhageNeural regeneration researchAugust 1, 2023Qu J, Zong HF, Shan Y, Zhang SC, Guan WP, Yang Y, Zhao HL.SAB4500631, RRID: AB_10743042), rabbit polyclonal anti-GRP78 antibody (1:1000, Abcam, Cambridge, UK, Cat# ab21685, RRID: AB_2119834), rabbit monoclonal anti-Bax antibody (1:2000, Abcam, Cat# ab182733), rabbit monoclonal

(20) Knockdown of ELF4 aggravates renal injury in ischemia/reperfusion mice through promotion of pyroptosis, inflammation, oxidative stress, and endoplasmic reticulum stressBMC molecular and cell biologyJuly 20, 2023Li L, Wang S, Wang W.primary antibodies against ELF4, Nrf2, NQO-1, GRP78, KIM-1, and IL-6 (1:1000, ab96075, ab137550, ab34173, ab21685, ab302932, and ab259341, Abcam, USA); IL-1β (1:1000, sc-12742, Santa Cruz Biotechnology); IL-18 and

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(1)

12/8/2022Excellent Anti-GRP78 BiP Antibody Kanika JainUsed this antibody to investigate the expression of GRP78 in the human and mice platelets following induction of ER stress.
(1)

11/18/2016GRP78 Expression In Melanoma Cells Rani OjhaTo understand the role of stress responses that promote resistance to targeted therapies in melanoma.

Anti-GRP78 BiP antibody from Abcam

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Anti-GRP78 BiP antibody from Abcam

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